How to Draw a Straight Line in Graphpad

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The preferred analysis and graphing solution purpose-built for scientific research. Join the world's leading scientists and discover how you can use Prism to save time, make more appropriate analysis choices, and elegantly graph and present your scientific research.

Biostatistics in the Life Sciences

GraphPad Prism was originally developed for experimental biologists, medicine scientists and pharmacologists. Meanwhile Graphpad Prism is used throughout the life sciences sector. Many undergraduate and graduate students also use GraphPad Prism.

GraphPad Prism guides you through the process of the analysis. It facilitates the choice of the statistical tests and help you to interpret the results. No other software offers curve fitting and other statistical analysis to be so easy, comprehensive, correct and simple!

Wide Range of Applications

GraphPad Prism provides t-tests, one, two, and three-way ANOVA, non-parametric comparisons, linear and non-linear regression, analysis of contingency tables and survival analysis. In addition, GraphPad Prism offers a large selection of presentable graphs. The graphics engine is extremely flexible and admits various designs. Flexible templates allow rapid creation of graphs with just a single click.

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• Trainings
• System Requirements
• Features

Discover What's New in Prism!

Enhanced Data Visualization and Graphs Customization

Bubble Plots

Create Bubble Plots directly from raw data, encoding variables for position (X- and Y-coordinates), color, and size

Violin plots

Visualize distributions of large data sets with either extended or truncated violin plots

Estimation Plots

Automatically display your analysis results

Smoothing spline

Major improvements in showing general data trends through Akima splines and smoothing splines with improved control over the number of knots, or inflection points

Improved Graphing and Customization Options

Stars on Graph

Automatically add multiple comparison results to graphs

Automatically label bar graphs

Annotate your bar graphs with values for the means, medians, or sample sizes to emphasize what's important in your work

Improved grouped graphs

Easily create graphs that show both individual points (scatter) along with bars for mean (or median) and error bars

And.....

More Intuitive Navigation

Now Featuring Eight Kinds of Data Tables

More Sophisticated Statistical Analyses

GraphPad Prism : Comprehensive Analysis and Powerful Statistics, Simplified

Organize Your Data Effectively

Unlike spreadsheets or other scientific graphing programs, Prism has eight different types of data tables specifically formatted for the analyses you want to run. This makes it easier to enter data correctly, choose suitable analyses, and create stunning graphs.

Perform The Right Analysis

Avoid statistical jargon. In clear language, Prism presents an extensive library of analyses from common to highly specific—nonlinear regression, t tests, nonparametric comparisons, one-, two- and three-way ANOVA, analysis of contingency tables, survival analysis, and much more. Each analysis has a checklist to help you understand the required statistical assumptions and confirm you have selected an appropriate test.

Get Actionable Help As You Go

Reduce the complexity of statistics. Prism's online help goes beyond your expectations. At almost every step, access thousands of pages from the online Prism Guides. Browse the Graph Portfolio and learn how to make a wide range of graph types. Tutorial data sets also help you understand why you should perform certain analyses and how to interpret your results.

Work Smarter, Not Harder

One-Click Regression Analysis

No other program simplifies curve fitting like Prism. Select an equation and Prism does the rest—fits the curve, displays a table of results and function parameters, draws the curve on the graph, and interpolates unknown values.

Focus on Your Research, Not Your Software

No coding required. Graphs and results are automatically updated in real time. Any changes to the data and analyses—adding missed data, omitting erroneous data, correcting typos, or changing analysis choices—are reflected in results, graphs, and layouts instantaneously.

Automate Your Work Without Programming

Reduce tedious steps to analyze and graph a set of experiments. It is easy to replicate your work by creating a template, duplicating a family, or cloning a graph—saving you hours of set up time. Apply a consistent look to a set of graphs with one click using Prism Magic.

The Fastest Way to Elegantly Graph and Share Your Work

Countless Ways to Customize Your Graphs

Focus on the story in your data, not manipulating your software. Prism makes it easy to create the graphs you want. Choose the type of graph, and customize any part—how the data is arranged, the style of your data points, labels, fonts, colors, and much more. The customization options are endless.

Export Publication-Quality Graphs With One Click

Reduce time to publish. Prism allows you to customize your exports (file type, resolution, transparency, dimensions, color space RGB/CMYK) to meet the requirements of journals. Set your defaults to save time.

Enhance Collaboration

Share more than your graphs. Prism's comprehensive record of your data enables effective collaboration with other scientists. All parts of your Prism project (raw data, analyses, results, graphs, and layouts) are contained in a single file that you can share with one click. Now others can easily follow your work at every step, enhancing the clarity of your findings and streamlining your collaborative efforts.

Discover What's New in Prism!

Introducing an even more powerful Prism featuring enhanced data visualization and graph customization, more intuitive navigation, and more sophisticated statistical analyses.

Enhanced Data Visualization

Violin plots

Visualize distributions of large data sets more clearly than with box-whisker or simple bar graphs

Subcolumn graphs

Organize related subsets of nested data in a single graph

Smoothing spline

Major improvements in showing general data trends through Akima splines and smoothing splines with improved control over the number of knots, or inflection points

No more smiles

More intelligent adjustments of data point positions in scatter plots for better looking graphs

Improved Graphing and Customization Options

Draw lines and brackets with centered text

Easily annotate your data with asterisks or custom labels

Automatically label bar graphs

Annotate your bar graphs with values for the means, medians, or sample sizes to emphasize what's important in your work

Improved grouped graphs

Easily create graphs that show both individual points (scatter) along with bars for mean (or median) and error bars

More Intuitive Navigation

Find related sheets easily

New family panel shows the family of sheets related to the current sheet, and chains of analyses are automatically indented

Easily navigate between multiple results tables

Analyses with multiple results tables now grouped into a single sheet with tabs for each result table; choose which tabs to show or hide

Improved Search

Search by sheets with highlights or notes of specified color

Now Featuring Eight Kinds of Data Tables

New: Multiple variables data table

Each row represents a different subject and each column is a different variable, allowing you to perform Multiple linear regression (including Poisson regression), extract subsets of data into other table types, or select and transform subsets of the data

New: Nested data table

Analyze and visualize data that contains subsets within related groups; Perform nested t tests and nested one-way ANOVA using data within these tables

More Sophisticated Statistical Analyses

Perform repeated measures ANOVA – even with missing data

Now Prism will automatically fit a mixed effects model to complete this analysis

Powerful Improvements in regular ANOVA

View cell, row, column, and grand means (or least square means when data is missing); test for homogeneity of variance. For one-way ANOVA, choose a test that does not assume homogeneous variances.

Nested t test and nested one-way ANOVA

Utilize new types of data tables to perform nested t-tests and nested ANOVA as well as multiple linear regression (including Poisson regression)

Graph residuals from multiple types of analyses

Test residuals for normality in four different ways, and choose from four different ways to display these residuals

Our GraphPad Prism Trainings

STATCON not only offers professional statistical software solutions, we also offer professional trainings and consulting for those solutions so that you get the maximum out of your software! We offer simple trainings that will introduce you to the software and it's basic features but also more comprehensive trainings with deepth inside into specific functions of the software. Trainings are held as In-house and Off-site trainings. If you would like to attend a comfortable online-training, take a look at our webinars.

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GraphPad Prism - Introduction

This training teaches a solid foundation for everyone working with GraphPad Prism. You learn the complete course of a data analysis ranging from data import and descriptive statistical analysis to creation and optimmization of the output. Go to Article...

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GraphPad Prism - Graph Creation

This one-day-course emphasizes the graphical features of Grapad PRISM. This course does not focus on any statistical details, only a few simple descriptive statistics (average, standard deviation, standard error) will be calculated using Graphpad Prism. This course is also used as an introduction of Graphpad Prism, thus other, more statistical, courses will built on it. Go to Article...

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GraphPad Prism - Means Comparison

This two-day-course is an advanced training based on the Introduction to Data Analysis with GraphPad Prism. It covers multiple statistical tests for group comparisons including ANOVA, t-Tests and nonparametric methods. Keywords like: t-test, ANOVA, Kruskal-Wallis, gaussian distribution, homogeneity, post hoc tests, paired / unpaired will be explained. Go to Article...

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GraphPad Prism - Regression

This two-day-course introduces linear and non-linear regression models. The class covers all methods of regression analysis (linear and nonlinear) which are implemented in GraphPad Prism. Keywords like: linearity, statistical modeling, least squares method, constraints, global fit, model comparisons, dose response curves and enzyme cinetic models will be explained. Go to Article...

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GraphPad Prism Webinar - Update Webinar

Since August 2012 there is a new release of Graphpad Prism. Not only the user interface is revamped, also many statistical and graphical functions were added. This webinar is a comprehensive and effective tour around the new version of PRISM Graphpad. Go to Article...

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GraphPad Prism Webinar (free) - Dose Response Regression

This websession presents a typical stepwise workflow beginning with data entry, briefly showing the different analysis procedures and finally presenting the results. Go to Article...

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GraphPad Prism Webinar (free) - Comparison of Means

offers a wide spectrum of statistical and graphical analysis. This websession presents a typical stepwise workflow beginning with data entry, briefly showing the different analysis procedures and finally presenting the results. Go to Article...

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System Requirements for the software GraphPad Prism

	Windows	Mac
Further Requirements	Must connect to the internet to verify the license when it is first activated and also at at least once every 30 days (or every 20 launches, whichever comes sooner).	Must connect to the internet to verify the license when it is first activated and also at at least once every 30 days (or every 20 launches, whichever comes sooner).
Operating System	Windows 7,8 and 10 (32-/64-Bit)	Runs under macOS X 10.9 (Mavericks) or later. If you use macOS 10.8, Prism will launch and seems to run OK, but we haven't tested Prism thoroughly under this version of macOS and can't provide much support. If you use 10.8, we urge you to update MacOS.
Display Resolution	at least 800 x 600 pixels	at least 1024 x 768 pixels
Disk Space	100 MB	130 MB

What's New in Prism 9 ?

Statistical comparisons

• Paired or unpaired t tests. Reports P values and confidence intervals.
• Automatically generate volcano plot (difference vs. P value) from multiple t test analysis.
• Nonparametric Mann-Whitney test, including confidence interval of difference of medians.
• Kolmogorov-Smirnov test to compare two groups.
• Wilcoxon test with confidence interval of median.
• Perform many t tests at once, using False Discovery Rate (or Bonferroni multiple comparisons) to choose which comparisons are discoveries to study further.
• Ordinary or repeated measures ANOVA followed by the Tukey, Newman-Keuls, Dunnett, Bonferroni or Holm-Sidak multiple comparison tests, the post-test for trend, or Fisher's Least Significant tests.
• One-way ANOVA without assuming populations with equal standard deviations using Brown-Forsythe and Welch ANOVA, followed by appropriate comparisons tests (Games-Howell, Tamhane T2, Dunnett T3)
• Many multiple comparisons test are accompanied by confidence intervals and multiplicity adjusted P values.
• Greenhouse-Geisser correction so repeated measures one-, two-, and three-way ANOVA do not have to assume sphericity. When this is chosen, multiple comparison tests also do not assume sphericity.
• Kruskal-Wallis or Friedman nonparametric one-way ANOVA with Dunn's post test.
• Fisher's exact test or the chi-square test. Calculate the relative risk and odds ratio with confidence intervals.
• Two-way ANOVA, even with missing values with some post tests.
• Two-way ANOVA, with repeated measures in one or both factors. Tukey, Newman-Keuls, Dunnett, Bonferroni, Holm-Sidak, or Fisher's LSD multiple comparisons testing main and simple effects.
• Three-way ANOVA (limited to two levels in two of the factors, and any number of levels in the third).
• Analysis of repeated measures data (one-, two-, and three-way) using a mixed effects model (similar to repeated measures ANOVA, but capable of handling missing data).
• Kaplan-Meier survival analysis. Compare curves with the log-rank test (including test for trend).
• Comparison of data from nested data tables using nested t test or nested one-way ANOVA (using mixed effects model).

Nonlinear Regression

• Fit one of our 105 built-in equations, or enter your own. Now including family of growth equations: exponential growth, exponential plateau, Gompertz, logistic, and beta (growth and then decay).
• Enter differential or implicit equations.
• Enter different equations for different data sets.
• Global nonlinear regression – share parameters between data sets.
• Robust nonlinear regression.
• Automatic outlier identification or elimination.
• Compare models using extra sum-of-squares F test or AICc.
• Compare parameters between data sets.
• Apply constraints.
• Differentially weight points by several methods and assess how well your weighting method worked.
• Accept automatic initial estimated values or enter your own.
• Automatically graph curve over specified range of X values.
• Quantify precision of fits with SE or CI of parameters. Confidence intervals can be symmetrical (as is traditional) or asymmetrical (which is more accurate).
• Quantify symmetry of imprecision with Hougaard's skewness.
• Plot confidence or prediction bands.
• Test normality of residuals.
• Runs or replicates test of adequacy of model.
• Report the covariance matrix or set of dependencies.
• Easily interpolate points from the best fit curve.
• Fit straight lines to two data sets and determine the intersection point and both slopes.

Column statistics

• Calculate descriptive statistics: min, max, quartiles, mean, SD, SEM, CI, CV, skewness, kurtosis.
• Mean or geometric mean with confidence intervals.
• Frequency distributions (bin to histogram), including cumulative histograms.
• Normality testing by four methods (new: Anderson-Darling).
• Lognormality test and likelihood of sampling from normal (Gaussian) vs. lognormal distribution.
• Create QQ Plot as part of normality testing.
• One sample t test or Wilcoxon test to compare the column mean (or median) with a theoretical value.
• Identify outliers using Grubbs or ROUT method.
• Analyze a stack of P values, using Bonferroni multiple comparisons or the FDR approach to identify "significant" findings or discoveries.

Linear regression and correlation

• Calculate slope and intercept with confidence intervals
• Force the regression line through a specified point.
• Fit to replicate Y values or mean Y.
• Test for departure from linearity with a runs test.
• Calculate and graph residuals in four different ways (including QQ plot).
• Compare slopes and intercepts of two or more regression lines.
• Interpolate new points along the standard curve.
• Pearson or Spearman (nonparametric) correlation.
• Multiple linear regression (including Poisson regression) using the new multiple variables data table.

Clinical (diagnostic) lab statistics

• Bland-Altman plots
• Receiver operator characteristic (ROC) curves
• Deming regression (type ll linear regression)

Simulations

• Simulate XY, Column or Contingency tables.
• Repeat analyses of simulated data as a Monte-Carlo analysis.
• Plot functions from equations you select or enter and parameter values you choose.

Other Calculations

• Area under the curve, with confidence interval.
• Transform data.
• Normalize.
• Identify outliers.
• Normality tests.
• Transpose tables.
• Subtract baseline (and combine columns).
• Compute each value as a fraction of its row, column or grand total.

How to Draw a Straight Line in Graphpad

Source: https://www.statcon.de/shop/en/software/graphics/graphpad-prism

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